Evaluation of antioxidant capacity of SAC DPPH Free Radical Scavenging Capacity Assay Kit (Nanjing Jiancheng Bioengineering Institute, A153-1-1, Nanjing, China): Prepare the working solution according to the kit instructions, mix with different concentrations of SAC solution, incubate in the dark at 25 for 30 min, centrifuge at 4000 rpm for 5 min, transfer 100L to a cuvette, zero with 80% methanol, and measure absorbance at 517 nm
Garlic oil upregulated the activity of various antioxidant enzymes and expression of pro-apoptotic effectors like Bax and Caspase-3 and downregulated the expression of the anti-apoptotic genes -arrestin-2, Bcl-2, and Bcl-X (76)
FIGURE 6 We further tested whether the biological factors of sex or age influenced baseline GSH concentrations
This combination therapy may inhibit tumor cell epithelial-mesenchymal transition via blocking CXCR2/TGF--mediated PI3K/Akt/GSK-3/Snail signaling pathway, suggesting potential for clinical trials in lung cancer patients [177]
In-gel digestion for MS analysis The Coomassie Brilliant Blue stained gel band was excised into small pieces and washed in order with water, 50 mM NH 4 HCO 3 in 50% acetonitrile and 100% acetonitrile
Archived from the original on 2018-10-22
