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The method illustrated is based on the reaction of GSH with the thiol reagent DTNB (5,5-dithiobis(2-nitrobenzoic acid)) to form GSSG and TNB (5-thionitrobenzoic acid), which is detected spectrophotometrically at 412 nm ( Giustarini et al

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Collectively, current clinical studies suggest that natural flavonoids, particularly quercetin, silymarin, EGCG, and grape seed proanthocyanidins, may exert therapeutic effects in both topical and systemic interventions for AD, with generally favorable safety profiles

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