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After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples

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Many genes or molecules have been identified to play pivotal roles in the regulation of APAP hepatotoxicity, so they are suggested to be potential targets for therapeutic intervention against APAP-induced liver injury

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