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Quality assessment and risk of bias In line with PRISMA guidelines, the assessment of methodological quality was conducted to evaluate the strength of evidence, recognising that methodological limitations may introduce bias

The samples were incubated on ice and centrifuged to separate the serum, which was then collected, transferred to clean tubes, and stored until further experimentation took place [2]

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For both human testis tissues and cultured TM3 cells, immunofluorescence and immunohistochemical staining were performed to visualize protein expression and localization

These typically improve as the body adjusts to the medication