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Afterwards cells were washed in RPMI 1640 (Life Technologies, without Glucose) and resuspended in RPMI 1640 (without Glucose, 100 M 2-NBDG (Life Technologies), 10% dialyzed FCS)

Arlington: AOAC International

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Wnt signaling can be subdivided into two types: -catenin-dependent signaling (the canonical pathway) and -catenin-independent signaling (the non-canonical pathway), with the latter further divided into the Wnt/planar cell polarity (PCP) and Wnt/calcium(Ca 2+ ) pathways (234)

As this neural hyperactivity persists, it increases the likelihood that tinnitus will become chronic