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Multiplexed CRISPR/Cas9-mediated metabolic engineering of -aminobutyric acid levels in Solanum lycopersicum

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At the core of the ERMS experiment is the collection of an array of collision-activated decomposition tandem mass spectra (CAD-MS-MS) of a molecular precursor ion, each obtained at a defined value of collision energy, to observe the fall of the intensity of the precursor and the gradual rise of the intensity of the generated fragments

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Reverse-transcription PCR (rtPCR) cDNA was generated using the Transcriptor First Strand cDNA Synthesis Kit (Roche)