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KG-dependent dioxygenases CRISPR library construction We constructed a pooled sgRNA library containing 64 sgRNAs targeting various genes whose enzymes require KG as a co-factor for their activity in addition to controls targeting intragenic regions as described previously (Joung et al., 2017) ( Table S1 )

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Protein quality control is also targeted by TLR4 signaling, which inhibits 20S/26S proteasome activity while modulating Akt/FoxO3a and p38 MAPK pathways