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Here's a quick reference table to illustrate these options: Ultimately, the choice is driven by your specific research design

For the analysis of biological benchmark data and the kinase inhibitor screen, the Spectronaut-reported normalized intensity data matrices were transformed into phosphorylation site tables with the plugin peptide collapse script using localization cutoff of 0.75

doi: 10.1523/JNEUROSCI.5855-12.2013 104 KnightR.CallewaertC.MarotzC.HydeE

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