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The supernatant layer was filtered with a 0.45 m syringe adapter to ensure a clear solution with no micro particles that may clog the HPLC column or syringe and affect the separation and 20 L was injected in triplicates in the chromatographic system

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Figure 5 Gene expression analysis revealed that T H 17-related genes are highly sensitive to SFN To get an unbiased view on differential mRNA expression profiles in untransformed T-cells after co-stimulation in the presence vs