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Critically, the GSH/GSSG redox couple maintains the thiol groups of structural and enzymatic proteins in their reduced, functional state preventing irreversible oxidative cross-linking that would impair protein function

doi: 10.1016/j.celrep.2016.05.009 26

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Crucially, the researchers noted that one month after stopping the supplement, glutathione levels returned to baseline, proving that continuous, sustained intake is required to maintain the therapeutic benefits

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