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crenulata and its active metabolites can inhibit the NF-B/NLRP3 pathway to reduce HH-induced brain oxidative stress damage, inflammatory responses, and BBB damage, which are attributed to improved energy metabolism and the anti-inflammatory M2 microglial phenotype (Jiang et al., 2022)

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Target sequence was selected by CRISPR-PLANT ( All the vectors were transformed into Agrobacterium tumefaciens strain GV3101 using electroporation