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The ITT was conducted one week following the GTT, and rats were injected intraperitoneally with insulin (0.75 U/kg)
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Calculations Plasma glucose rate of appearance ( R a ) and rate of glycerol production were calculated under near steady-state conditions (30 to 0 min) using the average enrichment of [6,6- 2 H 2 ]glucose and [ 2 H 5 ]glycerol or [1,2,3- 13 C 3 ]glycerol, with conventional isotope dilution calculations as previously described [22]
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