Results and discussion Massive changes in gene expression define the physiological state of biofilm and floating cells To study the transition of cells from floating to biofilm and its further progression, we used static cultures and developed the experimental setup summarized in Supplementary Fig
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An equal volume of dye Mastermix (1 M JC-1, 0.005% digitonin, 10 g ul 1 oligomycin, 5 mM -mercaptoethanol in DTEP buffer) was added, and after 10 min at room temperature, the cells were added on top of the peptide template at a concentration of 40,000 cells per well