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We then transfected cultured myotubes with siRNA against AKT or with non-targeting (scrambled) siRNA to test the effects of L-carnitine on TNF--induced FOXO3a, p70S6K, MAFbx and MuRF1 expression in the absence of AKT

This approach can help sensitive individuals titrate more gradually, reducing the shock of jumping from one full dose level to the next

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