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Cytotoxicity in human cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays using two different cell lines (HeLa and HepG2), which revealed half-maximum inhibitory concentrations (IC 50 ) values above 1 mM for all compounds, enabling a sufficient efficacy window for further treatment of bacterial infections in vivo (Supplementary Table 3 and Supplementary Fig

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Knockout mutants for GGT1 show a phenotype of decreased growth and earlysenescence, linked to accumulation of GSSG (Ohkamu-Ohtsu et al., 2007a)

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