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In conclusion, we demonstrated in the first half of Figure 1 that pre-treatment with GSSSG or LASSS effectively prevents nitration of HGF-Y198, Y250, thereby preserving the ability of HGF to activate myogenic stem satellite cells in vitro through the c-met signaling pathway (panels b-d and panel e left column)

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But once water is added, freezing becomes destructive

The spin trap and other reagents (peroxidase substrate, S 0 species and H 2 O 2 ) were added as described in the figure legends to a final volume of 50 l