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with Protein A/G agarose beads (Santa Cruz Biotechnology) for 1 h at 4 C

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Others changed dose or switched to saline vehicle to mitigate irritation

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As the GP activity was previously studied only in tissues such as rat liver and placenta without optimization of substrata conditions [7], the selection of blood as the target compartment to study the effect of systemic peroxide levels has the advantage of investigating the compartment subjected to all the comprehensive effects of peroxide systemic accumulation