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Therefore, therapeutic strategies that target the distinct mitochondrial metabolic features of CSCs and DTPs, such as disrupting the AMPKOXPHOS axis, inhibiting mitophagy, or decoupling metabolic signaling from DNA repair, represent a promising approach to overcoming chemoresistance in CRC

Finally, the mixture was centrifuged at 18,000 rpm for 10 min, and 100 L supernatant was collected for LCMS/MS analysis