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234,235,236,237 Unfortunately, tumor cells often remodel the tumor immune microenvironment through various mechanisms to achieve immune evasiveness, 238 such as reducing tumor antigen presentation, decreasing the infiltration or function of tumor-inhibitory immune cells, and promoting the infiltration of immunosuppressive cells

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Pancreatic cancer ACLY KO cells were then transduced with a retroviral pOZ-N vector (addgene 3781) expressing ACLY or an empty pOZ-N vector to generate cells with reconstituted ACLY (ACLY KO plus ACLY cDNA) or an empty vector (ACLY KO)