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Method Validation Validation of the presented method regarding specificity, linearity and lower limit of quantification was performed in rat plasma, fecal homogenates, urine and bile, according to the FDA guidelines for validation of bioanalytical methods ( The specificity was assessed by analyzing and comparing the chromatograms of blank plasma, fecal homogenates, the urine and bile with the corresponding spiked samples and study samples after dosing

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