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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C
Oral 'hydrogen water' induces neuroprotective ghrelin secretion in mice
10.1016/J.FOODCONT.2017.02.044 Food Control 77 JouanyJ
6 The ferroptosis-immune-metabolic axis in asthma The chronic inflammation and structural remodeling that characterize asthma are sustained through a dynamic interplay between ferroptosis, immune activation, and metabolic reprogramming
To quantify fluorescence intensity, images of S100 and GFAP staining were converted to binary files, with ROIs defined to encompass astrocytes showing positive staining
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