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Intriguingly, this study regarding elevated glutamine metabolism in cancer cells also showed that glutaminolysis could in turn reinforce metabolic reprogramming, thus implying that glutamine metabolism plays a crucial role in tumorigenesis and tumor progression 16 (Fig

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Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)

They also found that GSH content was decreased by 50% of the control when the cells were treated with uranyl nitrate (Hao et al., 2014)

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