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Single-Cell Gel Electrophoresis (Comet Assay) The comet assay procedure was performed under alkaline conditions (26, 27)

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More importantly, oral administration of these compounds leads to their accumulation in the brain, heart, muscle and liver mitochondria (Ross et al., 2005)

Total proteins from lysed cells or patient tissue were extracted using RIPA lysis buffer and electrophoresed by SDS-PAGE and transferred onto PVDF membranes subsequently

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