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Previous research has established that VDR is expressed in endothelial cells, vascular smooth muscle cells, and macrophages within the vascular wall being involved in involved in vascular homeostasis [13, 47]

Tetramethyl ethylene diamine (100 L, 100%, TEMED, Acros) was added and vortexing continued for another minute

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Five flasks were used to dissolve various interfering biochemicals

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The rest of the paracetamol (510% of an ingested dose) is oxidised in the liver, to a highly reactive and toxic free radical called N-acetyl-p-benzoquinone imine (NAPQI)