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343,1075 Supplementing with pyruvate, a key link between glycolysis and the TCA cycle, improves brain energy status

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Though ROS are important for cell signaling, immune function, and defence against pathogens (38), oxidative stress can be produced by either excessive ROS generation or insufficient antioxidant defence mechanisms (2)

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Electroporation After a 1-week acclimatization, cells were collected at a density of 1 10 6 cells and mixed with plasmid diluted in Opti-MEM reduced serum medium (Thermo Fisher Scientific)