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& Siuzdak, G

intracellular cysteine: **p = 0.0053, *p = 0.0212

Never use: Tap water (bacteria, contaminants) Distilled water (no preservative) Saline solution (wrong osmolarity for some peptides) Only bacteriostatic water

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Optional NAD+ Support An optional add-on is NAD+ or Nicotinamide Adenine Dinucleotide

Most of the research into coffee enemas derives from case studies, the majority of which highlight the risks of the practice