Recombinant proteins were separated using Bolt 412% Bis-Tris or NuPAGE 38% Tris-Acetate gels and transferred to nitrocellulose membranes with the iBlot 2 Dry Blotting System (all Thermo Fisher Scientific)
We generated four groups because the phenomenology of stress, anxiety, and depression share symptoms (Ramn-Arbus et al., 2020) and are also a reference for possible comorbidities, which are common
This selectivity allows GH stimulation without significant activation of stress-related hormone pathways
Shelf life: 24 months under the conditions above and in its original packaging
References Garvey, W.T., Batterham, R.L., Bhatta, M., Buscemi, S., et al
Both card types are accepted, and a letter of medical necessity is available on request to support reimbursement claims