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Children generally require weight-adjusted regimens, often 100 mcg monthly after initial loading

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After centrifugation (15 min, 12,000 g, 4 C), the upper aqueous phase was carefully collected and transferred into a new tube containing 1 ml of freshly prepared 70% ethanol solution, mixed by pipetting, and loaded onto a RNeasy spin column (RNeasy mini kit, Qiagen)

3A), PF4 C re Thbs1 flox KO mice exhibited higher lung bacterial burden at day 1 post-infection compared to WT littermate controls (Fig