A total of 350 g of whole cell lysates from H69 cells with or without HGF stimulation was used for each KPSS phosphoantibody array screen, which is an antibody-based method (Kinetworks) that relies on sodium dodecyl sulphate (SDS)-polyacrylamide minigel electrophoresis and multilane immunoblotters to permit the specific and quantitative detection of numerous protein kinases (PK) or other signal transduction proteins simultaneously (Pelech et al, 2003)
Statistical analysis GraphPad Prism (9.0.0) was used for data analysis
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Cartridges were pre-conditioned with 2% acetic acid (AA) in methanol (2 ml) and water (2 ml), and equilibrated with 1 ml 2% AA in water
Unlike earlier oral formulations that require strict fasting protocols for absorption, Orforglipron is designed to be absorbed more efficiently, offering a more convenient daily dosing schedule
