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Differentiation of myoblasts into myotubes was induced when the cells had achieved 9095% confluence by switching the medium from GM to differentiation medium consisting of DMEM supplemented with 2% horse serum (5 days), then treated as described in the figures

10.3389/fncel.2021.615372 553 ZhangY.SwandaR

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Step 3: After the injection After the injection, you can follow these steps: Gently remove the needle at the same angle at which you inserted it

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