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Human mast cell heterogeneity: Histamine release from mast cells dispersed from skin, lung, adenoids, tonsils, and colon in response to IgE-dependent and nonimmunoiogic stimuli

Volume depends on your target dose

Cell cytotoxic death was assessed using a CytoTox-Glo Cytotoxicity assay (Promega Corporation, Madison, WA, USA) according to the manufacturer's protocols

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Therefore, sample pretreatment (5% SSA treatment) should be performed quickly