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S2.4 Comparison between HH curves, pHp, and amount of niclosamide in solution To complete this theoretical assessment, plotted in Figure S5 (red symbols) is the pHp curve, giving the maximum amount of niclosamide expected to be in solution (uM) versus the solution pH (right axis) using the values derived from experiment of pKa = 6.87, S o = 1.77uM

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For AOD 9604, the fasted morning window is more strictly important due to the insulin interaction that can blunt the lipolytic effect

Solution Compatibility

What matters is the resulting concentration: extremely dilute solutions (under ~0.5 mg/mL) require larger injection volumes per dose, which becomes uncomfortable and can shift absorption kinetics