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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

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10.1371/journal.pone.0028378 39 HalliwellB

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ACS Sens 2:857875 Aldewachi H, Chalati T, Woodroofe MN, Bricklebank N, Sharrack B, Gardinera P (2018) Gold nanoparticle-based colorimetric biosensors

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(2) GHK-Cu is a peptide complex consisting of the tripeptide GHK (glycine, histidine, and lysine), bound to a divalent copper ion (Cu)

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