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Briefly, 100 age-matched male and female flies were segregated in separate vials and transferred to fresh vials during the experimental period every five days
doi: 10.3390/nu12040951, 110
Hoechst 33342 (4 g mL 1 ) was then added and incubated at 37 C for 5 min to stain the cell nuclei
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Bruising and discomfort have also been reduced as a result of newer technology
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