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Andrews N, Stowe J, Kirsebom F, Toffa S, Rickeard T, Gallagher E, et al

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Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication

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so the brain is protected from Se deficiency (Zhang et al., 2008)

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