Chumakov et al., 2021
After three 5-min washes in PBS, sections were incubated with the corresponding secondary antibody for 45 min
The binding of HGF to c-met was detected with biotinylated anti-HGF polyclonal Ab (1:500 dilution in the 0.1% BSA-TTBS for 3 h at 25C), TACS streptavidin-HRP conjugate (1:1600 dilution for 20 min), and TMB substrate solution
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USA 112 , 87208725 (2015)