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Clinical Features Polyuria, polydipsia, polyphagia, and weight loss Nausea, vomiting, abdominal pain Dry mouth, sunken eyes, tachycardia, hypotension, Deep, rapid breathing (Kussmaul respiration) to compensate for acidosis Characteristic fruity (acetone) odor on breath Drowsiness, confusion, and in severe cases, coma E

The CRISPRi-dCpf1 system uses inactivated Cpf1 (dCpf1), which does not generate DSBsthis avoids the lethal issue caused by the low homologous recombination efficiency of Clostridia and achieved 3- to 100-fold gene silencing efficiency in 27 Clostridium strains

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