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MAMs were collected with a Pasteur pipette and then diluted ten times with a buffer containing 250 mM mannitol, 5 mM HEPES (pH 7.4) and 0.5 mM EGTA

This combination is designed for comprehensive research into tissue regeneration, inflammatory response modulation, wound healing, and extracellular matrix remodeling

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Women also have higher numbers of CD4 + T cells [101], and estrogen increases the expression of interferon (IFN)-, an important cytokine for immune activation [102, 103]