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Subsequently, the experiments were evaluated by measuring the amount of TMA released into the culture medium ( For the inhibition assay, 1,996 L of fecal slurry was placed in the wells of a deep-well plate, and 2 L of fermentation products solution diluted in sterile water was added at the beginning of the experiment (T0) to obtain a final concentration of 50 M (or 7.99 10 3 mg/mL)

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Furthermore, coffee can contribute to stomach irritation and anxiety whilst you are hungover