It is proposed that the C* domain located at the C-terminus of CesAand the TE domain located at the C-terminus of CesB works as a cooperative esterification and depsipeptide elongation center, repeatedly recruiting the action of the C* domain to oligomerize tetradepsipeptides to give octa- and dodecadepsipeptides prior to macrocyclization by the TE domain
the DIC/DMAPDIC/HOBtDIC/HOAtBOP/HOBtBOP/HOAtHBTU/HOBtHBTU/HOAtHATU/HOBtHATU/HOAtHCTU/HOBtHUTC/HOAtTBTU/HOBt()TBTU/HOBt78759895 Although theoretically there are DIC/DMAP or DIC/HOBt or DIC/HOAt or BOP/HOBt or BOP/HOAt or HBTU/HOBt or HBTU/HOAt or HATU/HOBt or HATU/HOAt or HCTU/HOBt or HUTC/HOAt or TBTU/ Reagents such as HOBt can be chosen, and theoretically there is no major difference
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