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This hypothesis is supported by post-mortem evidence of reduced pyruvate dehydrogenase activity in the brains of individuals with ASD (Gu et al., 2013), which may impair the conversion of pyruvate to acetyl-CoA, leading to mitochondrial dysfunction and accumulation of lactate and alanine

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PCR conditions were as following: initial denaturation at 95 C for 5 min, followed by 37 cycles of denaturation (94 C for 40 s), annealing (57 C for 30 s) and extension (72 C for 40 s), with the final extension at 72 C for 10 min

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Jama Pediatr