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Cell viability was assessed by Trypan Blue exclusion using an automated cell counter

The absorbance of the resultant mixture was determined at 325 nm for a duration of 5 min with 30 s interval, employing a UV/VIS spectrophotometer (UV-1280, Shimadzu, Kyoto, Japan) [26]

Parental burden and its correlates in families of children with autism spectrum disorder: a multicentre study with two comparison groups