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Colloids Surf B: Biointerfaces (2023) 234:113676

Our protocol reference pages for the two common vial formats the GHK-Cu 50 mg vial dosage protocol and the GHK-Cu 100 mg vial dosage protocol set out the same reconstitution mathematics against each vials specific handling considerations, and carry the same evidentiary caveats

The slides were washed in PBS and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

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Allow the alcohol to air dry for 5 to 10 seconds

However, in evaluating the skin parameters, the addition of HA did not improve the results compared with collagen and vitamin C together