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Hemin showed negligible effect on the expression of most factors such as MAT1A

The main fermentation was conducted in 100 mL of YCK50D medium (Cha et al., 2004) containing 30 g/L yeast extract, 5 mM cysteine, 0.6 g/L KH 2 PO 4 , and 50 g/L glucose

Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min

DOI.org (Crossref) , Sports Medicine (Auckland, N.Z.) , vol