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The membranes were probed with primary antibodies at a 1:1000 dilution and secondary HRP-conjugated antibodies at a 1:2000 dilution

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"Ethanol effects in a rat hepatoma cell line: induction of gamma-glutamyltransferase"

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Depending on the existence of concurrent diseases, serum chemistry profiles and other diagnostics may also be re-evaluated at least every six months