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Intestinal transit test: After 4 weeks of temperature housing, mice were fasted for 2 h and administered a 300 L oral gavage of 6% w/v carmine red solution in 0.5% w/v methyl cellulose, and food was available ad libitum for the remainder of the experiment 41

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Pro-inflammatory cytokines produced by adipose tissue support tumor-promoting intercellular crosstalk in the tumor microenvironment [14], thus enhancing tumor cell progression, angiogenesis, and invasion as essential requirements for metastasis [15]