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A Bradford assay (Thermo Scientific) was used to determine the total protein concentration, with ovalbumin as the protein standard

Therefore, the NMDA receptors in granule cells are not essential for enrichment-induced increase in survival of new neurons, although we cannot exclude the possibility that there is a sub-component of a survival-inducing effect which is dependent on the NMDA receptors

It is found and made in every cell of the body, but occurs in higher concentrations in the heart, muscle tissue and liver

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