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Recent studies have shown that inducing ferroptosis could be an innovative strategy to target aggressive cancers, especially those with a drug- tolerant persister phenotype [17,18,19,20,21,22,23], but it remains challenging to identify biomarkers that could distinguish cancer cells that are inherently susceptible to ferroptosis [24]

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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for one hour at room temperature

After five minutes, initiate the reaction by adding 20 L of the Cumene Hydroperoxide Solution into each well and mix thoroughly (it is recommended to use a multi-channel pipette)

Without enough B12, things can go wrong, sometimes seriously, especially if deficiency is not recognised and treated

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