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Then, FA was applied in the indicated concentrations and the animals were incubated for another 4 h, followed by the quantification of survival right after the treatment

Seino, S

A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)

This assay is limited in the identification of subtypes other than 1a and 1b, mixed infections, recombinant and/or novel strains 25

Health Drug Benefits 10 (4), 178188 (2017)

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