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Shrunken mitochondria with condensed ruptured outer membranes as well as vestigial cristae are a physical hallmark of ferroptosis [24]

The cells were then treated six more times with 35 M

Sometimes letting it sit for several hours between shakes

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Additionally, treatment with 10 nM E2, for 7 days, stimulated differentiation to the neuronal lineage, and inhibited differentiation to astrocytes (Zhang et al., 2019)